What is the purpose of trimming the bottom corners of TLC plates? The 2019 Stack Overflow...
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What is the purpose of trimming the bottom corners of TLC plates?
The 2019 Stack Overflow Developer Survey Results Are InDiffusion/Streaky bands on TLC plateHow to translate the mobile phase from thin layer chromatography to column?What mechanisms might make a substance travel different distances on the same TLC plate?How to interpret TLC data regarding the purity of one’s sampleSuitable first-year experiment idea - stability of coordination complexes in TLC?How does the polarity of the eluent and sample affect the Rf value in thin layer chromatography?Quantitative information from silica gel TLC plate?In which pH range are silica-based TLC plates stable?How to prepare and store anisaldehyde tlc staining solution? What will be the color of this solution? What is its shelf life?Transesterification of vegetable oils to produce biofuels - problems in the labVisualization of TLC plates
$begingroup$
During my chemistry lab course, I was told by my instructor to make 2 small cuts on the bottom corners of the TLC (Thin-Layer Chromatography) plate. Does this make the solvent rise uniformly on the plate? Is this a standard practice in labs? I could not find any information online. In this question the OP has made the cuts as well, but this does not seem to make the solvent rise uniformly.
experimental-chemistry chromatography
edited yesterday
orthocresol♦
40.1k7116247
asked yesterday
Apoorv PotnisApoorv Potnis
699423
$endgroup$
|
show 1 more comment
$begingroup$
During my chemistry lab course, I was told by my instructor to make 2 small cuts on the bottom corners of the TLC (Thin-Layer Chromatography) plate. Does this make the solvent rise uniformly on the plate? Is this a standard practice in labs? I could not find any information online. In this question the OP has made the cuts as well, but this does not seem to make the solvent rise uniformly.
experimental-chemistry chromatography
edited yesterday
orthocresol♦
40.1k7116247
asked yesterday
Apoorv PotnisApoorv Potnis
699423
$endgroup$
$begingroup$
My guess is so you will remember which is the bottom of the plate. I cannot see how it would improve flow in any significant way.
$endgroup$
– Night Writer
yesterday
$begingroup$
Never heard of this. Certainly not std practice in any lab I've worked in.
$endgroup$
– Waylander
yesterday
$begingroup$
Re: comments, It’s quite a personal thing. Plenty of people I know do it, plenty don’t.
$endgroup$
– orthocresol♦
yesterday
$begingroup$
@orthicresol I know plenty of people that don't operate correctly. This is totally another story. One can get decent results, nevertheless. I know lot of people using ematocrito capillaries to deposite on the plate. It does not make it handbook practice, nor it should recommended. Of course preparing ad hoc capillaries is tedious and requires a higher than usual flame temperature, so many go with far too big capillaries. Etc.
$endgroup$
– Alchimista
yesterday
$begingroup$
@Alchimista What are ematocrito capillaries? Googling seems of no use. I use glass capillaries with a smaller diameter than melting point capillaries.
$endgroup$
– Apoorv Potnis
yesterday
|
show 1 more comment
$begingroup$
During my chemistry lab course, I was told by my instructor to make 2 small cuts on the bottom corners of the TLC (Thin-Layer Chromatography) plate. Does this make the solvent rise uniformly on the plate? Is this a standard practice in labs? I could not find any information online. In this question the OP has made the cuts as well, but this does not seem to make the solvent rise uniformly.
experimental-chemistry chromatography
edited yesterday
orthocresol♦
40.1k7116247
asked yesterday
Apoorv PotnisApoorv Potnis
699423
$endgroup$
During my chemistry lab course, I was told by my instructor to make 2 small cuts on the bottom corners of the TLC (Thin-Layer Chromatography) plate. Does this make the solvent rise uniformly on the plate? Is this a standard practice in labs? I could not find any information online. In this question the OP has made the cuts as well, but this does not seem to make the solvent rise uniformly.
experimental-chemistry chromatography
experimental-chemistry chromatography
edited yesterday
orthocresol♦
40.1k7116247
asked yesterday
Apoorv PotnisApoorv Potnis
699423
edited yesterday
orthocresol♦
40.1k7116247
asked yesterday
Apoorv PotnisApoorv Potnis
699423
edited yesterday
orthocresol♦
40.1k7116247
edited yesterday
orthocresol♦
40.1k7116247
edited yesterday
orthocresol♦
40.1k7116247
40.1k7116247
asked yesterday
Apoorv PotnisApoorv Potnis
699423
asked yesterday
Apoorv PotnisApoorv Potnis
699423
asked yesterday
Apoorv PotnisApoorv Potnis
699423
699423
$begingroup$
My guess is so you will remember which is the bottom of the plate. I cannot see how it would improve flow in any significant way.
$endgroup$
– Night Writer
yesterday
$begingroup$
Never heard of this. Certainly not std practice in any lab I've worked in.
$endgroup$
– Waylander
yesterday
$begingroup$
Re: comments, It’s quite a personal thing. Plenty of people I know do it, plenty don’t.
$endgroup$
– orthocresol♦
yesterday
$begingroup$
@orthicresol I know plenty of people that don't operate correctly. This is totally another story. One can get decent results, nevertheless. I know lot of people using ematocrito capillaries to deposite on the plate. It does not make it handbook practice, nor it should recommended. Of course preparing ad hoc capillaries is tedious and requires a higher than usual flame temperature, so many go with far too big capillaries. Etc.
$endgroup$
– Alchimista
yesterday
$begingroup$
@Alchimista What are ematocrito capillaries? Googling seems of no use. I use glass capillaries with a smaller diameter than melting point capillaries.
$endgroup$
– Apoorv Potnis
yesterday
|
show 1 more comment
$begingroup$
My guess is so you will remember which is the bottom of the plate. I cannot see how it would improve flow in any significant way.
$endgroup$
– Night Writer
yesterday
$begingroup$
Never heard of this. Certainly not std practice in any lab I've worked in.
$endgroup$
– Waylander
yesterday
$begingroup$
Re: comments, It’s quite a personal thing. Plenty of people I know do it, plenty don’t.
$endgroup$
– orthocresol♦
yesterday
$begingroup$
@orthicresol I know plenty of people that don't operate correctly. This is totally another story. One can get decent results, nevertheless. I know lot of people using ematocrito capillaries to deposite on the plate. It does not make it handbook practice, nor it should recommended. Of course preparing ad hoc capillaries is tedious and requires a higher than usual flame temperature, so many go with far too big capillaries. Etc.
$endgroup$
– Alchimista
yesterday
$begingroup$
@Alchimista What are ematocrito capillaries? Googling seems of no use. I use glass capillaries with a smaller diameter than melting point capillaries.
$endgroup$
– Apoorv Potnis
yesterday
$begingroup$
My guess is so you will remember which is the bottom of the plate. I cannot see how it would improve flow in any significant way.
$endgroup$
– Night Writer
yesterday
$begingroup$
My guess is so you will remember which is the bottom of the plate. I cannot see how it would improve flow in any significant way.
$endgroup$
– Night Writer
yesterday
$begingroup$
Never heard of this. Certainly not std practice in any lab I've worked in.
$endgroup$
– Waylander
yesterday
$begingroup$
Never heard of this. Certainly not std practice in any lab I've worked in.
$endgroup$
– Waylander
yesterday
$begingroup$
Re: comments, It’s quite a personal thing. Plenty of people I know do it, plenty don’t.
$endgroup$
– orthocresol♦
yesterday
$begingroup$
Re: comments, It’s quite a personal thing. Plenty of people I know do it, plenty don’t.
$endgroup$
– orthocresol♦
yesterday
$begingroup$
@orthicresol I know plenty of people that don't operate correctly. This is totally another story. One can get decent results, nevertheless. I know lot of people using ematocrito capillaries to deposite on the plate. It does not make it handbook practice, nor it should recommended. Of course preparing ad hoc capillaries is tedious and requires a higher than usual flame temperature, so many go with far too big capillaries. Etc.
$endgroup$
– Alchimista
yesterday
$begingroup$
@orthicresol I know plenty of people that don't operate correctly. This is totally another story. One can get decent results, nevertheless. I know lot of people using ematocrito capillaries to deposite on the plate. It does not make it handbook practice, nor it should recommended. Of course preparing ad hoc capillaries is tedious and requires a higher than usual flame temperature, so many go with far too big capillaries. Etc.
$endgroup$
– Alchimista
yesterday
$begingroup$
@Alchimista What are ematocrito capillaries? Googling seems of no use. I use glass capillaries with a smaller diameter than melting point capillaries.
$endgroup$
– Apoorv Potnis
yesterday
$begingroup$
@Alchimista What are ematocrito capillaries? Googling seems of no use. I use glass capillaries with a smaller diameter than melting point capillaries.
$endgroup$
– Apoorv Potnis
yesterday
|
show 1 more comment
1 Answer
1
active
oldest
votes
$begingroup$
Yes, your question contains the answer. Think about capillarity.
Without the little cuts the eluant would be "sucked" and flow from both the bottom and the left ( and right) edges of the thin plate, resulting in a tilted flow that
tends to move the elute faster along the vertical edges while clumping the spots to the center;
decrease the resolution at the given conditions by deforming and enlarging the spots.
Think of it as a weird bad executed two dimensionally-developed plate, if it helps.
Note that beside resolution, the cuts should be there to guarantee a levelled horizontal and straight flow front, as requisite to obtain reliable and useful ratio front values.
(Good practice: draw the real appearance of the developed plate or even post it to the lab journal, so that a future user get a feeling of the separation whatever it happened. The latter not recommended if a product spots out to be irritant/allergenic).
Left: correct - Right: not horizontal flow frontline due to capillarity at vertical edges.
As OP mentions an example with cutted corners but not levelled flow, obviously cutting the corners but plunging the plate in too much eluant is useless. The idea is to have eluant sucked up just by the bottom edge.
answered yesterday
AlchimistaAlchimista
2,0721310
$endgroup$
$begingroup$
Well I've seen the light now! Never heard of this trick. Surprised it would matter. Is it considered standard practice?
$endgroup$
– Night Writer
yesterday
2
$begingroup$
It should be standard practice! Still, a not perfect plate might be of some help, of course. Sometimes you do the same over and over for instance. But a Rf value shouldn't take into accoubt how far from center a compound/mixture was dropped.
$endgroup$
– Alchimista
yesterday
$begingroup$
@Night Writer ↑
$endgroup$
– Alchimista
yesterday
$begingroup$
Would help if TLC plates were sold like this. Cutting your own plates is a good way to have a reportable H&S incident.
$endgroup$
– Waylander
yesterday
$begingroup$
Scissors marketing perhaps :) @Waylander. More seriously, normally TLC prepared plates come in a rather large size, or at least these are cheaper, and then one cuts them in the meaningful size.
$endgroup$
– Alchimista
yesterday
|
show 8 more comments
Your Answer
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1 Answer
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1 Answer
1
active
oldest
votes
active
oldest
votes
active
oldest
votes
$begingroup$
Yes, your question contains the answer. Think about capillarity.
Without the little cuts the eluant would be "sucked" and flow from both the bottom and the left ( and right) edges of the thin plate, resulting in a tilted flow that
tends to move the elute faster along the vertical edges while clumping the spots to the center;
decrease the resolution at the given conditions by deforming and enlarging the spots.
Think of it as a weird bad executed two dimensionally-developed plate, if it helps.
Note that beside resolution, the cuts should be there to guarantee a levelled horizontal and straight flow front, as requisite to obtain reliable and useful ratio front values.
(Good practice: draw the real appearance of the developed plate or even post it to the lab journal, so that a future user get a feeling of the separation whatever it happened. The latter not recommended if a product spots out to be irritant/allergenic).
Left: correct - Right: not horizontal flow frontline due to capillarity at vertical edges.
As OP mentions an example with cutted corners but not levelled flow, obviously cutting the corners but plunging the plate in too much eluant is useless. The idea is to have eluant sucked up just by the bottom edge.
answered yesterday
AlchimistaAlchimista
2,0721310
$endgroup$
$begingroup$
Well I've seen the light now! Never heard of this trick. Surprised it would matter. Is it considered standard practice?
$endgroup$
– Night Writer
yesterday
2
$begingroup$
It should be standard practice! Still, a not perfect plate might be of some help, of course. Sometimes you do the same over and over for instance. But a Rf value shouldn't take into accoubt how far from center a compound/mixture was dropped.
$endgroup$
– Alchimista
yesterday
$begingroup$
@Night Writer ↑
$endgroup$
– Alchimista
yesterday
$begingroup$
Would help if TLC plates were sold like this. Cutting your own plates is a good way to have a reportable H&S incident.
$endgroup$
– Waylander
yesterday
$begingroup$
Scissors marketing perhaps :) @Waylander. More seriously, normally TLC prepared plates come in a rather large size, or at least these are cheaper, and then one cuts them in the meaningful size.
$endgroup$
– Alchimista
yesterday
|
show 8 more comments
$begingroup$
Yes, your question contains the answer. Think about capillarity.
Without the little cuts the eluant would be "sucked" and flow from both the bottom and the left ( and right) edges of the thin plate, resulting in a tilted flow that
tends to move the elute faster along the vertical edges while clumping the spots to the center;
decrease the resolution at the given conditions by deforming and enlarging the spots.
Think of it as a weird bad executed two dimensionally-developed plate, if it helps.
Note that beside resolution, the cuts should be there to guarantee a levelled horizontal and straight flow front, as requisite to obtain reliable and useful ratio front values.
(Good practice: draw the real appearance of the developed plate or even post it to the lab journal, so that a future user get a feeling of the separation whatever it happened. The latter not recommended if a product spots out to be irritant/allergenic).
Left: correct - Right: not horizontal flow frontline due to capillarity at vertical edges.
As OP mentions an example with cutted corners but not levelled flow, obviously cutting the corners but plunging the plate in too much eluant is useless. The idea is to have eluant sucked up just by the bottom edge.
answered yesterday
AlchimistaAlchimista
2,0721310
$endgroup$
$begingroup$
Well I've seen the light now! Never heard of this trick. Surprised it would matter. Is it considered standard practice?
$endgroup$
– Night Writer
yesterday
2
$begingroup$
It should be standard practice! Still, a not perfect plate might be of some help, of course. Sometimes you do the same over and over for instance. But a Rf value shouldn't take into accoubt how far from center a compound/mixture was dropped.
$endgroup$
– Alchimista
yesterday
$begingroup$
@Night Writer ↑
$endgroup$
– Alchimista
yesterday
$begingroup$
Would help if TLC plates were sold like this. Cutting your own plates is a good way to have a reportable H&S incident.
$endgroup$
– Waylander
yesterday
$begingroup$
Scissors marketing perhaps :) @Waylander. More seriously, normally TLC prepared plates come in a rather large size, or at least these are cheaper, and then one cuts them in the meaningful size.
$endgroup$
– Alchimista
yesterday
|
show 8 more comments
$begingroup$
Yes, your question contains the answer. Think about capillarity.
Without the little cuts the eluant would be "sucked" and flow from both the bottom and the left ( and right) edges of the thin plate, resulting in a tilted flow that
tends to move the elute faster along the vertical edges while clumping the spots to the center;
decrease the resolution at the given conditions by deforming and enlarging the spots.
Think of it as a weird bad executed two dimensionally-developed plate, if it helps.
Note that beside resolution, the cuts should be there to guarantee a levelled horizontal and straight flow front, as requisite to obtain reliable and useful ratio front values.
(Good practice: draw the real appearance of the developed plate or even post it to the lab journal, so that a future user get a feeling of the separation whatever it happened. The latter not recommended if a product spots out to be irritant/allergenic).
Left: correct - Right: not horizontal flow frontline due to capillarity at vertical edges.
As OP mentions an example with cutted corners but not levelled flow, obviously cutting the corners but plunging the plate in too much eluant is useless. The idea is to have eluant sucked up just by the bottom edge.
answered yesterday
AlchimistaAlchimista
2,0721310
$endgroup$
Yes, your question contains the answer. Think about capillarity.
Without the little cuts the eluant would be "sucked" and flow from both the bottom and the left ( and right) edges of the thin plate, resulting in a tilted flow that
tends to move the elute faster along the vertical edges while clumping the spots to the center;
decrease the resolution at the given conditions by deforming and enlarging the spots.
Think of it as a weird bad executed two dimensionally-developed plate, if it helps.
Note that beside resolution, the cuts should be there to guarantee a levelled horizontal and straight flow front, as requisite to obtain reliable and useful ratio front values.
(Good practice: draw the real appearance of the developed plate or even post it to the lab journal, so that a future user get a feeling of the separation whatever it happened. The latter not recommended if a product spots out to be irritant/allergenic).
Left: correct - Right: not horizontal flow frontline due to capillarity at vertical edges.
As OP mentions an example with cutted corners but not levelled flow, obviously cutting the corners but plunging the plate in too much eluant is useless. The idea is to have eluant sucked up just by the bottom edge.
answered yesterday
AlchimistaAlchimista
2,0721310
edited yesterday
answered yesterday
AlchimistaAlchimista
2,0721310
answered yesterday
AlchimistaAlchimista
2,0721310
answered yesterday
AlchimistaAlchimista
2,0721310
2,0721310
$begingroup$
Well I've seen the light now! Never heard of this trick. Surprised it would matter. Is it considered standard practice?
$endgroup$
– Night Writer
yesterday
2
$begingroup$
It should be standard practice! Still, a not perfect plate might be of some help, of course. Sometimes you do the same over and over for instance. But a Rf value shouldn't take into accoubt how far from center a compound/mixture was dropped.
$endgroup$
– Alchimista
yesterday
$begingroup$
@Night Writer ↑
$endgroup$
– Alchimista
yesterday
$begingroup$
Would help if TLC plates were sold like this. Cutting your own plates is a good way to have a reportable H&S incident.
$endgroup$
– Waylander
yesterday
$begingroup$
Scissors marketing perhaps :) @Waylander. More seriously, normally TLC prepared plates come in a rather large size, or at least these are cheaper, and then one cuts them in the meaningful size.
$endgroup$
– Alchimista
yesterday
|
show 8 more comments
$begingroup$
Well I've seen the light now! Never heard of this trick. Surprised it would matter. Is it considered standard practice?
$endgroup$
– Night Writer
yesterday
2
$begingroup$
It should be standard practice! Still, a not perfect plate might be of some help, of course. Sometimes you do the same over and over for instance. But a Rf value shouldn't take into accoubt how far from center a compound/mixture was dropped.
$endgroup$
– Alchimista
yesterday
$begingroup$
@Night Writer ↑
$endgroup$
– Alchimista
yesterday
$begingroup$
Would help if TLC plates were sold like this. Cutting your own plates is a good way to have a reportable H&S incident.
$endgroup$
– Waylander
yesterday
$begingroup$
Scissors marketing perhaps :) @Waylander. More seriously, normally TLC prepared plates come in a rather large size, or at least these are cheaper, and then one cuts them in the meaningful size.
$endgroup$
– Alchimista
yesterday
$begingroup$
Well I've seen the light now! Never heard of this trick. Surprised it would matter. Is it considered standard practice?
$endgroup$
– Night Writer
yesterday
$begingroup$
Well I've seen the light now! Never heard of this trick. Surprised it would matter. Is it considered standard practice?
$endgroup$
– Night Writer
yesterday
2
2
$begingroup$
It should be standard practice! Still, a not perfect plate might be of some help, of course. Sometimes you do the same over and over for instance. But a Rf value shouldn't take into accoubt how far from center a compound/mixture was dropped.
$endgroup$
– Alchimista
yesterday
$begingroup$
It should be standard practice! Still, a not perfect plate might be of some help, of course. Sometimes you do the same over and over for instance. But a Rf value shouldn't take into accoubt how far from center a compound/mixture was dropped.
$endgroup$
– Alchimista
yesterday
$begingroup$
@Night Writer ↑
$endgroup$
– Alchimista
yesterday
$begingroup$
@Night Writer ↑
$endgroup$
– Alchimista
yesterday
$begingroup$
Would help if TLC plates were sold like this. Cutting your own plates is a good way to have a reportable H&S incident.
$endgroup$
– Waylander
yesterday
$begingroup$
Would help if TLC plates were sold like this. Cutting your own plates is a good way to have a reportable H&S incident.
$endgroup$
– Waylander
yesterday
$begingroup$
Scissors marketing perhaps :) @Waylander. More seriously, normally TLC prepared plates come in a rather large size, or at least these are cheaper, and then one cuts them in the meaningful size.
$endgroup$
– Alchimista
yesterday
$begingroup$
Scissors marketing perhaps :) @Waylander. More seriously, normally TLC prepared plates come in a rather large size, or at least these are cheaper, and then one cuts them in the meaningful size.
$endgroup$
– Alchimista
yesterday
|
show 8 more comments
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My guess is so you will remember which is the bottom of the plate. I cannot see how it would improve flow in any significant way.
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– Night Writer
yesterday
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Never heard of this. Certainly not std practice in any lab I've worked in.
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– Waylander
yesterday
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Re: comments, It’s quite a personal thing. Plenty of people I know do it, plenty don’t.
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– orthocresol♦
yesterday
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@orthicresol I know plenty of people that don't operate correctly. This is totally another story. One can get decent results, nevertheless. I know lot of people using ematocrito capillaries to deposite on the plate. It does not make it handbook practice, nor it should recommended. Of course preparing ad hoc capillaries is tedious and requires a higher than usual flame temperature, so many go with far too big capillaries. Etc.
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– Alchimista
yesterday
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@Alchimista What are ematocrito capillaries? Googling seems of no use. I use glass capillaries with a smaller diameter than melting point capillaries.
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– Apoorv Potnis
yesterday